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Team:Paris-Saclay/Project/Notebook/Week 4
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | ||
__NOTOC__ | __NOTOC__ | ||
| + | *We place an order for the primers | ||
| + | *DNA (biobricks) Resuspension using iGEM protocol | ||
| + | *Transformation of each biobricks by thermal shock to have a large quantity of DNA | ||
| + | *Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid | ||
| + | *We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep | ||
| + | *Miniprep to purify each biobrick (ready to use) | ||
| + | *Groove for two colonies because they did not grown on the liquid culture | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Revision as of 16:40, 26 September 2012
- We place an order for the primers
- DNA (biobricks) Resuspension using iGEM protocol
- Transformation of each biobricks by thermal shock to have a large quantity of DNA
- Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid
- We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep
- Miniprep to purify each biobrick (ready to use)
- Groove for two colonies because they did not grown on the liquid culture
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