Team:HKU HongKong/Data/Weekly Notebook.html

From 2012.igem.org

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<font face="Trebuchet MS" size="6"> Daily Lab Notebook</font></span></h2>
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<font face="Trebuchet MS" size="4">Weeks 1&2:</font></span></h2>
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1)</span><span style="color: rgb(35, 35, 35); font-family: Lato, Tahoma, Arial, sans-serif; font-size: 13px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); display: inline !important; float: none; ">
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</span><font face="Tahoma" size="2"><b> Amplification of pvdQ gene using PCR from Pseudomonas aeuriginosa genomic DNA. The designed primers contain the prefix and suffix so that typical digestion for insertion into iGEM’s standard vector can be subsequently performed.</b></font><br>
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  <br>
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  <br>
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  </span><span style="font-size: 10pt; font-family: Tahoma">2)</span><font color="#232323" size="2" face="Tahoma"><b>Test digestion of amplified pvdQ with EcoRI only, EcoRI & SpeI, PstI, and EcoRI & XbaI. </b></font></h2>
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&nbsp;</font></p>
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  <font size="2" face="Tahoma" color="#232323">&nbsp;</font><font color="#232323"><span style="font-size: 10pt; font-family: Tahoma"><br>
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</span><b><font size="2" face="Tahoma">3). Constitutive </font></b>
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<span style="font-size: 10pt; font-family: Tahoma">Promoter</span><b><font size="2" face="Tahoma">
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(pLacI) + Ribosomal Binding Site + LuxR Gene + Double Terminator + LuxR Promoter
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(J09855) + Ribosomal Binding Site (B0034) + pvdQ Gene + Double Terminator
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(B0015)</font></b></font></p>
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<p class="MsoNormal" style="text-align: left"><b>
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  <font size="2" face="Tahoma" color="#232323">4). </font>
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  <font size="2" face="Tahoma" color="#232323">LuxR Promoter (R1062) + Ribosomal
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    Binding Site, LuxR Gene, Double Terminator, &amp; Ribosomal Binding Site (amplified
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from K137076) + pvdQ Gene + Double Terminator (B0015)</font></b></p>
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Revision as of 22:23, 26 September 2012

Team:HKU Hong Kong - 2012

Team:HKU HK

From 2011.igem.org