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Team:Tokyo-NoKoGen/Project/modelling
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Revision as of 12:53, 26 September 2012
Tokyo-NoKoGen
2012
- overview
- lux operon
- rhodopsin
- modelling
- Future work
- project
Modeling
We aimed to build the mathematical model using MATLAB R2012b SymBiology (http://www.mathworks.com/). However, since we had no knowledge and no sufficient data, so we could not complete the modeling.
For building the mathematical model of lux operon, we investigated about reaction mechanism of lux operon. Figure.1 is described reaction of Lux operon.
Primary reaction equations are bellow:
(a) FMNH2 + O2 + RCHO -> FMN + H2O + RCOOH + Light
LuxAB, a luciferase, catalyzes the reaction (a). RCHO is substrate of luciferase (luciferin).
(b) FMN + NADPH + H+ -> FMNH2 + NADP+
This is FMN reduction pathway. By this reaction, It becomes possible to reuse FMN produced in reaction (a). The reaction is operated via a “Ping-pong Bi-bi” mechanism.
(d) RCOOH + ATP + luxE-luxC -> PPi + AMP + luxE-luxC-RCO
(e) luxE-luxC-RCO + NADPH -> RCHO + luxE-luxC + NADP+
LuxE and luxC involve in the decanal recycling. The recycling pathway makes luminous bacteria possible to emit light without substrate from outside.
We aimed to build the mathematical model using MATLAB R2012b SymBiology (http://www.mathworks.com/). However, since we had no knowledge and no sufficient data, so we could not complete the modeling.
For building the mathematical model of lux operon, we investigated about reaction mechanism of lux operon. Figure.1 is described reaction of Lux operon.
Primary reaction equations are bellow:
(a) FMNH2 + O2 + RCHO -> FMN + H2O + RCOOH + Light
LuxAB, a luciferase, catalyzes the reaction (a). RCHO is substrate of luciferase (luciferin).
(b) FMN + NADPH + H+ -> FMNH2 + NADP+
This is FMN reduction pathway. By this reaction, It becomes possible to reuse FMN produced in reaction (a). The reaction is operated via a “Ping-pong Bi-bi” mechanism.
(d) RCOOH + ATP + luxE-luxC -> PPi + AMP + luxE-luxC-RCO
(e) luxE-luxC-RCO + NADPH -> RCHO + luxE-luxC + NADP+
LuxE and luxC involve in the decanal recycling. The recycling pathway makes luminous bacteria possible to emit light without substrate from outside.
