Team:Korea U Seoul/Project/Improvement

     Rice Guardian project was to build an engineered E. coli which detects and kills. However, our team had difficulties making plasmid construction which follows iGEM regulation. Therefore, our team decided to make Xanthomonas oryzae KACC10331 detecting E. coli beforehand. As we already discussed the mechanism of these bacteria, our team used red light emitting protein (RFP) as a final output instead of lysis and bacteriocin gene. Our alternative approach was successful and we decided to go one step further.Our teamis conducting an experiment to add lysis and bacteriocin gene and this experiment will be done before iGEM championship.

     Addition of lysis and bacteriocin gene ensures death of nearby Xanthomonas oryzae KACC10331. Bacteriocins are proteinaceoustoxins produced by bacteria to inhibit the growth of similar or closely related bacterial strain(s). They are typically considered to be narrow spectrum antibiotics, though this has been debated . Lysis gene causes death of E. coli releasing bacteriocin inside the cell.

     Constitutive promoter will produce RaxR and RaxH. Protein synthesized from the genes allows cell to detect Ax21. When RaxR and RaxH proteins detect Ax21, They work as protein kinase (mechanism unknown) and stimulate bacteriocin and lysis gene. When lysis protein induces cell death of the E. coli , bacteriocin will burst into the surrounding and attacks Xanthomonas oryzae KACC10331.

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