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Team:Exeter/lab book/3gip/wk11
From 2012.igem.org
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<p><b><u>Monday 15th September</u></b></p> | <p><b><u>Monday 15th September</u></b></p> | ||
| - | <p><u><i>Disaster!</i></u> I had not heat deactivated the enzymes after digestion so I had to perform the <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:# | + | <p><u><i>Disaster!</i></u> I had not heat deactivated the enzymes after digestion so I had to perform the <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#57b947"><u>transfer of the BioBricks</u></a> into pSB1C3 again. A repeat of Fridays lab but the ligation was left overnight in the fridge. </p><br> |
<p><b><u>Tuesday 16th September</u></b></p> | <p><b><u>Tuesday 16th September</u></b></p> | ||
Revision as of 15:28, 26 September 2012
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The 3-Gene Inducible Plasmid: 17th - 21st September 2012 Monday 15th September Disaster! I had not heat deactivated the enzymes after digestion so I had to perform the transfer of the BioBricks into pSB1C3 again. A repeat of Fridays lab but the ligation was left overnight in the fridge. Tuesday 16th September Today I was handing the lab work over to Alex. Please see Single Gene Plasmids and Enzyme Characterisation: 24th - 28th September 2012 |
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