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Team:SDU-Denmark/labwork/Notebook/week8
From 2012.igem.org
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<p><b>20-08-2012 to 26-08-2012</b></p></br> | <p><b>20-08-2012 to 26-08-2012</b></p></br> | ||
| + | <h2> Miniprep and Nanodrop</h2> | ||
<p>We made a gel of the SST 1 from earlier and cut out the slice.</br> | <p>We made a gel of the SST 1 from earlier and cut out the slice.</br> | ||
We did miniprep on the liquid cultures from the day before (SST 2 and FFT 9) and found out that there was no product of use from the FFT vial (bacteria must have grown badly) but excellent product from SST 2 (105,3 ηg/μL first time and 82,7 ηg/μL the second time) which was marked N1 and N2. </br> | We did miniprep on the liquid cultures from the day before (SST 2 and FFT 9) and found out that there was no product of use from the FFT vial (bacteria must have grown badly) but excellent product from SST 2 (105,3 ηg/μL first time and 82,7 ηg/μL the second time) which was marked N1 and N2. </br> | ||
| - | N1 was the first run of miniprep product and the N2 was the second. We made a gel where we ran the N1 SST | + | N1 was the first run of miniprep product and the N2 was the second. We made a gel where we ran the N1 SST and cut out the smallest slice of the 2 fragments (approx. 1900 bp). The DNA was extracted form the gel.</br></br> |
| - | We | + | We made a new miniprep on the liquid cultures from the day before (FFT 9) and found out that there were still no product of use from the FFT vial. With concentration of only 4.0ηg/μL and 2.9ηg/μL in nanodrop. Therefore we made a new liquid culture of FFT9.</br> |
| - | We ran nanodrop on the SST PCR from yesterday and got about 412-420 ηg/μL | + | We ran nanodrop on the SST PCR from yesterday and got about 412-420 ηg/μL concentration. </br> |
| - | The liquid cultures form | + | The liquid SST cultures form earlier was taken out of the incubator, (after approximately 47 hours) and put in the refrigerator.</br></p> |
Revision as of 11:57, 26 September 2012
Laboratory Notebook
20-08-2012 to 26-08-2012
Miniprep and Nanodrop
We made a gel of the SST 1 from earlier and cut out the slice. We did miniprep on the liquid cultures from the day before (SST 2 and FFT 9) and found out that there was no product of use from the FFT vial (bacteria must have grown badly) but excellent product from SST 2 (105,3 ηg/μL first time and 82,7 ηg/μL the second time) which was marked N1 and N2. N1 was the first run of miniprep product and the N2 was the second. We made a gel where we ran the N1 SST and cut out the smallest slice of the 2 fragments (approx. 1900 bp). The DNA was extracted form the gel. We made a new miniprep on the liquid cultures from the day before (FFT 9) and found out that there were still no product of use from the FFT vial. With concentration of only 4.0ηg/μL and 2.9ηg/μL in nanodrop. Therefore we made a new liquid culture of FFT9. We ran nanodrop on the SST PCR from yesterday and got about 412-420 ηg/μL concentration. The liquid SST cultures form earlier was taken out of the incubator, (after approximately 47 hours) and put in the refrigerator.
