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Team:LMU-Munich/Data/crustpromoters
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| - | <p align="justify">The figure below shows the result of the evaluation of the three promoters for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. Each promoter (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) has two independent clones and both were measured three times. To be able to know in which growth phase the cells are the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. | + | <p align="justify">The figure below shows the result of the evaluation of the three promoters for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. Each promoter (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) has two independent clones and both were measured three times. To be able to know in which growth phase the cells are the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p> |
| - | {| style="color:black;" cellpadding="3" width=" | + | {| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;" |
| style="width: 70%;background-color: #EBFCE4;" | | | style="width: 70%;background-color: #EBFCE4;" | | ||
{| | {| | ||
| - | |[[File:Promotoren.png|Protein distribution in spore coat of ''Bacillus subtilis''| | + | |[[File:Promotoren.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px|center]] |
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| style="width: 70%;background-color: #EBFCE4;" | | | style="width: 70%;background-color: #EBFCE4;" | | ||
| - | {| style="color:black;" cellpadding="0" width=" | + | {| style="color:black;" cellpadding="0" width="90%" cellspacing="0" border="0" align="center" style="text-align:left;" |
|style="width: 70%;background-color: #EBFCE4;" | | |style="width: 70%;background-color: #EBFCE4;" | | ||
| - | <font color="#000000"; size="2">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub>. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone. </font> | + | <font color="#000000"; size="2"><p align="justify">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub>. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font> |
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Revision as of 10:10, 26 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Crust promoter measurements
The figure below shows the result of the evaluation of the three promoters for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. Each promoter (PcgeA, PcotV, PcotYZ) has two independent clones and both were measured three times. To be able to know in which growth phase the cells are the optical density (OD600) was measured throughout the whole experiment.
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