Team:Carnegie Mellon/Notebook/4 June 2012

From 2012.igem.org

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'''6/04'''
'''6/04'''
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** Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
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* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
** Harvested DH5-alpha cells from stock culture and made cells competent.
** Harvested DH5-alpha cells from stock culture and made cells competent.
** Followed transformation protocol from Dr. Doonan’s lab (see Protocols)
** Followed transformation protocol from Dr. Doonan’s lab (see Protocols)

Revision as of 20:49, 26 June 2012

6/04

  • Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
    • Harvested DH5-alpha cells from stock culture and made cells competent.
    • Followed transformation protocol from Dr. Doonan’s lab (see Protocols)