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Team:Colombia/Notebook/Journal
From 2012.igem.org
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For each one we obtained accesion numbers: | For each one we obtained accesion numbers: | ||
| - | -'' Arabidopsis thaliana'': | + | -'' Arabidopsis thaliana'': [http://www.ncbi.nlm.nih.gov/nuccore/AY099810.2 AY099810.2] |
| - | -'' Colletotrichum spp.'' : | + | -'' Colletotrichum spp.'' : [http://www.ncbi.nlm.nih.gov/nucest/GW342409.1 GW342409.1] |
| - | -'' Trichoderma spp.'' : | + | -'' Trichoderma spp.'' : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2] |
Revision as of 20:53, 26 June 2012
Chitinase
To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):
- Arabidopsis thaliana
- Colletotrichum spp.
- Trichoderma spp.
For each one we obtained accesion numbers:
- Arabidopsis thaliana: [http://www.ncbi.nlm.nih.gov/nuccore/AY099810.2 AY099810.2]
- Colletotrichum spp. : [http://www.ncbi.nlm.nih.gov/nucest/GW342409.1 GW342409.1]
- Trichoderma spp. : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]
Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. All of the sequences were suitable for use.
We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.
Primers
- Colletotrichum spp. : - Trichoderma spp. :
