Team:Colombia/Notebook/Journal

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== Chitinase  ==
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To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different '' Vibrio spp.'' was not suitable or the strain was found (''Alivibrio fischeri ES114'', ''Alivibrio fischeri M11''):
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-'' Arabidopsis thaliana''
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-'' Colletotrichum spp.''
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-'' Trichoderma spp.''
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For each one we obtained accesion numbers:
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-'' Arabidopsis thaliana'':
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-'' Colletotrichum spp.'' :
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-'' Trichoderma spp.''    :
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Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. '''All of the sequences were suitable for use'''.
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We selected ''Colletotrichum spp.'' and ''Trichoderma spp.'' sequences to design primers.
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'''Primers'''
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-'' Colletotrichum spp.'' :
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-'' Trichoderma spp.''    :

Revision as of 20:47, 26 June 2012

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Chitinase

To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):

- Arabidopsis thaliana

- Colletotrichum spp.

- Trichoderma spp.

For each one we obtained accesion numbers:

- Arabidopsis thaliana:

- Colletotrichum spp. :

- Trichoderma spp.  :


Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. All of the sequences were suitable for use.

We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.

Primers

- Colletotrichum spp. : - Trichoderma spp.  :

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