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Team:Penn/Notebook/DrugDelivery
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We digested mCherry PCR product with restriction enzymes BamHI and NotI. Then we column purified mCherry and ligated into NAS152 backbone. Afterwards we transformed NAS152-mCherry into DH5alpha cell lines. This was then poured 25 LB-Kan plates. | We digested mCherry PCR product with restriction enzymes BamHI and NotI. Then we column purified mCherry and ligated into NAS152 backbone. Afterwards we transformed NAS152-mCherry into DH5alpha cell lines. This was then poured 25 LB-Kan plates. | ||
| + | |||
| + | '''6/13/2012''' | ||
| + | |||
| + | '''Wet Lab''' | ||
| + | |||
| + | Unknown. Must consult lab notebook. | ||
| + | |||
| + | '''6/14/2012''' | ||
| + | |||
| + | '''Wet Lab''' | ||
| + | |||
| + | Same as above. | ||
| + | |||
| + | '''6/15/2012''' | ||
| + | |||
| + | '''Wet Lab''' | ||
| + | |||
| + | Once again. | ||
| + | |||
| + | '''Dry Lab''' | ||
| + | |||
| + | Met with Mark Goulian to develop the project. Also obtained pDawn and pDusk plasmids. | ||
Revision as of 20:35, 26 June 2012
Week 1
6/07/2012
Wet Lab
Today we transformed both versions of cph8 from 2012 Distribution.
Dry Lab
We also placed order for the following new BioBricks:
- BBa_K207001 - PhyB-DBD Fusion
- BBa_K422012 - Pif3 (Aar1 C part)
- BBa_K422013 - PhyB (Aar1 C part)
- BBa_K592006 - pFixK2
- BBa_K592004 - YF1
- BBa_K592005 - FixJ
- BBa_K592000 - Cph8
- BBa_K365000 - Pif3
In addition, we brainstormed and developed a new idea to use the PYP/GCN system to activate a gene.
6/08/2012
Wet Lab
No colonies appeared in the transformation of both cph8 a/b. We suspect that the failure of the transformatiosns were due to bad sequences.
Week 2
6/11/2012
Wet Lab
We performed PCR to obtain mCherry from the NAS 151 plasmid. Following this we ran a 1% gel and purified the product which resulted in a 30ns/λ yield.
6/12/2012
Wet Lab
We digested mCherry PCR product with restriction enzymes BamHI and NotI. Then we column purified mCherry and ligated into NAS152 backbone. Afterwards we transformed NAS152-mCherry into DH5alpha cell lines. This was then poured 25 LB-Kan plates.
6/13/2012
Wet Lab
Unknown. Must consult lab notebook.
6/14/2012
Wet Lab
Same as above.
6/15/2012
Wet Lab
Once again.
Dry Lab
Met with Mark Goulian to develop the project. Also obtained pDawn and pDusk plasmids.
