An overview on our system

Our favourite parts:

	BBa_K875001, T5 cumate operator: We have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity.
	BBa_K875003, CymR: We have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression.
	BBa_K875004, LPP-OmpA-scFv: We have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6.

The part we have improved:

BBa_K875020, Beta-Glucosidase (Osaka 2010):

This composite part is developed by previous work by UNITS iGEM team 2011 and Edinburgh team 2011. We improved and characterized this part demonstrating its efficiency.

Other parts that we developed:

BBa_K875002, T5 Lac Operator:

We have shown that this promoter is a very useful biobricks in the primary testing of protein expression thanks to his handiness.

BBa_K875005, LPP-OmpA-SIP:

We have realized this biobrick that is small and very stable at the same time. Thank to this high stability it is a valid alternative to the scFv.

BBa_K875006, PelB-scFv:

We have create this composit as an optimum system to secrete the protein inside the bacterial periplasm and also outside the outer mambrane according to the protein size.