Team:ZJU-China/sourcecode1.htm

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<body class="home">
<body class="home">
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<h2>Week 1 (06.22-06.24): Preparation</h2>
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<h3>Source program 1:</h3>
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<h3>June 22</h3>
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<p align="justify">data ribo;</p>
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<p align="justify">1. Do some washing.</p>
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<p align="justify">input gfp theo @@;</p>
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<p align="justify">2. Prepare LB plates, including ampicillin, kanamycin and spectinomycin resistance.</p>
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<p align="justify">cards;</p>
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<p align="justify">3. Get some E.coli DH5&alpha;competent cells from Prof. Xiao Mu. Receive E.coli BL21*(DE3) strain requested previously.</p>
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<p align="justify">22230 0 25855 0.1 29227 0.2 34357 0.3 34575 0.4 39766 0.5</p>
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<p align="justify">4. Transformation: extract plasmid pCJDD0 (Ampr), pCJDFA (Kanr) and pCJDFB (Spr) from filter paper received, transform them into DH5&alpha;competent cells respectively.</p>
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<p align="justify">14905 0 21407 0.1 21546 0.2 24699 0.3 27383 0.4 30470 0.5</p>
 +
<p align="justify">27774 0 28647 0.1 29131 0.2 29751 0.3 31131 0.4 46907 0.5</p>
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<p align="justify">；</p>
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<p align="justify">proc glm;</p>
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<p align="justify">class theo;</p>
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<p align="justify">model gfp=theo;</p>
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<p align="justify">Means theo/LSD;</p>
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<p align="justify">run;</p>
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<h3>June 23</h3>
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<img src="https://static.igem.org/mediawiki/igem.org/8/8f/Riboscaffold_SAS_1.jpg" width="700px" />
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<p align="justify">Amplify E.coli BL21*(DE3) strain.</p>
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<img src="https://static.igem.org/mediawiki/igem.org/7/7c/Riboscaffold_SAS_2.jpg" width="700px" />
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<h3>June 24</h3>
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<img src="https://static.igem.org/mediawiki/igem.org/5/5d/Riboscaffold_SAS_3.jpg" width="700px" />
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<p align="justify">1. Make glycerol stock of E.coli BL21*(DE3) strain.</p>
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<p align="justify">2. Make competent cells of E.coli BL21*(DE3) strain and DH10&beta;.</p>
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<p align="justify">White means no significant variations between two treatments of Theophylline and red means significant variations. </p>
 +
 
 +
<h3>Source program 2:</h3>
 +
 
 +
<p align="justify">data ribo;</p>
 +
<p align="justify">input gfp theo @@;</p>
 +
<p align="justify">cards;</p>
 +
<p align="justify">29188 0 30700 0.1 19999 0.3 23492 0.5 31119 0.7 15671 1</p>
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<p align="justify">13870 0 16483 0.2 16965 0.4 17516 0.5 16221 0.6</p>
 +
<p align="justify">；</p>
 +
<p align="justify">proc glm;</p>
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<p align="justify">class theo;</p>
 +
<p align="justify">model gfp=theo;</p>
 +
<p align="justify">Means theo/LSD;</p>
 +
<p align="justify">run;</p>
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<img src="https://static.igem.org/mediawiki/igem.org/4/4f/Riboscaffold_SAS_4.jpg" width="700px" />
</body></html>
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Revision as of 06:12, 26 September 2012

HOME

Source program 1:

data ribo;

input gfp theo @@;

cards;

22230 0 25855 0.1 29227 0.2 34357 0.3 34575 0.4 39766 0.5

14905 0 21407 0.1 21546 0.2 24699 0.3 27383 0.4 30470 0.5

27774 0 28647 0.1 29131 0.2 29751 0.3 31131 0.4 46907 0.5

；

proc glm;

class theo;

model gfp=theo;

Means theo/LSD;

run;

White means no significant variations between two treatments of Theophylline and red means significant variations.

Source program 2:

data ribo;

input gfp theo @@;

cards;

29188 0 30700 0.1 19999 0.3 23492 0.5 31119 0.7 15671 1

13870 0 16483 0.2 16965 0.4 17516 0.5 16221 0.6

；

proc glm;

class theo;

model gfp=theo;

Means theo/LSD;

run;

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