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Team:Colombia/Project/Experiments/Our Design
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=Our design= | =Our design= | ||
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| + | The purpose of our design is to generate genetically-modified bacteria with a "detect and alert" system. With this aim, we design two plasmids: | ||
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| + | -The first plasmid has two parts, one part is changeable and the other one is always present. The changeable part is the pathogen detection module that changes depending on the organism that is going to be detected. This year we have worked in the detection of [https://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces fungus (chitin detection)] or [https://2012.igem.org/Team:Colombia/Project/Experiments/Ralstonia Ralstonia spp. (3 OH-PAME detection)], so detection module has that two options. Furthermore, the detection turns on the second plasmid (Plux promoter) by generating a signal (LuxI-LuxR complex), doesn’t matter which pathogen we want to detect the signal generated is always same because this part doesn’t change. | ||
[[File:si.png|600px|thumb|center]] | [[File:si.png|600px|thumb|center]] | ||
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| + | -The second plasmid never changes because it´s aim is to alert the plant and ¨sleep¨ the bacteria. For alert the plant is produced salicylic acid that activates the salicylic acid defense (systemic defense) of the host plant and for sleep the bacterias, we introduced a [https://2012.igem.org/Team:Colombia/Project/Experiments/Pseudomonas toxin-antitoxin system (HipA-HipB)] that allows to reduce the quantity of active population when the pathogen is not detected. | ||
[[File:gen2.png|500px|thumb|center]] | [[File:gen2.png|500px|thumb|center]] | ||
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| + | Our design was made thanks to the use of [https://2012.igem.org/Team:Colombia/Modeling/Diff mathematical models] that helped us to check the best way if our design works and even it helped us to changed some parts from the first design proposed. | ||
Revision as of 05:04, 26 September 2012
Template:Https://2012.igem.org/User:Tabima
Our design
The purpose of our design is to generate genetically-modified bacteria with a "detect and alert" system. With this aim, we design two plasmids:
-The first plasmid has two parts, one part is changeable and the other one is always present. The changeable part is the pathogen detection module that changes depending on the organism that is going to be detected. This year we have worked in the detection of fungus (chitin detection) or Ralstonia spp. (3 OH-PAME detection), so detection module has that two options. Furthermore, the detection turns on the second plasmid (Plux promoter) by generating a signal (LuxI-LuxR complex), doesn’t matter which pathogen we want to detect the signal generated is always same because this part doesn’t change.
-The second plasmid never changes because it´s aim is to alert the plant and ¨sleep¨ the bacteria. For alert the plant is produced salicylic acid that activates the salicylic acid defense (systemic defense) of the host plant and for sleep the bacterias, we introduced a toxin-antitoxin system (HipA-HipB) that allows to reduce the quantity of active population when the pathogen is not detected.
Our design was made thanks to the use of mathematical models that helped us to check the best way if our design works and even it helped us to changed some parts from the first design proposed.
