Team:CINVESTAV-IPN-UNAM MX

From 2012.igem.org

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The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on ''R. sphaeroides'' photosynthesis cluster regulation.
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The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on ''R. palustris'' chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.
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Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.
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Revision as of 20:06, 26 September 2012

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Logo Principal

  • [home.htm Homeâ–¼]
  • [# Rhodofactoryâ–¼]
    • [Overview.htm Overviewâ–¼]
    • [Light Response.htm Light Responseâ–¼]
    • [Oxigen Response.htm Oxigen Responseâ–¼]
    • [Chassis.htm Chassisâ–¼]
    • [Perspective.htm Perspectiveâ–¼]
  • Resultsâ–¼
    • [Biobricks.htm Biobricksâ–¼]
    • [Notebook.htm Notebookâ–¼]
    • [Protocol.htm Protocolâ–¼]
    • [Light Response.htm Light Responseâ–¼]
    • [Oxigen Response.htm Oxigen Responseâ–¼]
  • [# IGEM Contributionâ–¼]
    • [Construction.htm Constructionâ–¼]
    • [Metods.htm Metodsâ–¼]
    • [Results.htm Resultsâ–¼]
  • [Modelling.htm Modellingâ–¼]
  • [# Teamâ–¼]
    • [Members.htm Membersâ–¼]
    • [Institute.htm Instituteâ–¼]
    • [Labs.htm Labsâ–¼]
    • [Sponsor.htm Sponsorâ–¼]
    • [Gallery.htm Galleryâ–¼]
    • [Acknowledgments.htm Acknowledgmentsâ–¼]
  • [# Outreachâ–¼]
    • [Human Practice.htm Human Practiceâ–¼]
    • [Safety.htm Safetyâ–¼]

File:Spacer.gif

Home 1.fw.png
RHODOFACTORY rojo-09.fw.png

The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on R. sphaeroides photosynthesis cluster regulation.

The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.

Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.

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