Team:Peking/Project/Phototaxis
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1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of <i>Escherichia coli</i>. <i>J. Bacteriol.</i>, 3:1307:1313 | 1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of <i>Escherichia coli</i>. <i>J. Bacteriol.</i>, 3:1307:1313 | ||
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Revision as of 10:25, 26 September 2012
Introduction
Bacterial phototaxis refers to the ability of bacteria to sense light changes in their extracellular environment and to bias their motility towards or away from the light. Phototactic responses are observed in many organisms such as Serratia marcescens, Tetrahymena, and Euglena. The behavior of phototaxis is the directed movement up a gradient to an increasing amount of light. This is analogous to positive chemotaxis; the usage of light as the attractant rather than chemicals.
![Figure](/wiki/images/c/c1/Peking2012_Home_Phototaxis.jpg)
Figure 1. Phototactic behavior of bacteria
For synthetic biology, phototaxis is a palace of mysteries because it has a rapid, precise response in comparison with chemotaxis while its mechanism has not been fully elucidated so far. As chemical diffused in the culture medium, chemotaxis has not the preciseness to origin of attractants. On the contrary, light is noninvasive, reversible and of good spatiotemparol control. With these advantages, phototaxis is more qualified for the application than chemotaxis in synthetic biology. Also, light signal is a potential link between the electrical components and bio-elements that endows phototaxis with a great potential of technology improvement such as light-guided cell targeting for medicine aggregation or tissue recovery in biomedical applications, light-induced bacterial enrichment or dispersion in environment protection and so forth.(Fig 1)
The model of chemotaxis pathway is quite clear and widely accepted. E.coli alternates between run(smooth swimming) and tumble, which result from distinct types of flagellar rotation, counter-clockwise(CCW) and clockwise(CW), respectively. A number of intracellular proteins provide the necessary signaling cascade which links the membrane receptors to the flagellar: CheY,CheZ,CheW, CheA,(Fig 2) etc.
![Figure 3.](/wiki/images/0/0f/Peking2012_pototatixs_circuit.png)
Figure 2. The simplified scheme of protein-protein interactions during chemotaxis of bacteria.
Among those proteins, CheY have the direct attachment to the flagella motors. Phosphorylated CheY diffuses through the cytoplasm to the motors,binding to FliM, a component of the C ring of the flagellar motor, and induces CW rotation of the motor. CheZ can dephosphorylate CheY, resulting in CCW rotations which leads to the smooth swimming. CheZ mutants result into swarming while CheY mutants are smooth-swimming.
CheZ is commonly used to affect bacterial motion in synthetic biology. Experiments shows that different levels of CheZ induced by arabinose can change the diameter of the swarming colony, combination between the quorum sensing part and CheZ can make bacteria form a stripe pattern. So we suppose combination between CheZ and our Luminesensor must have an effect on bacteria in motion. As modeling reveals, it does have a phototaxis phenomenon where light is a negative stimuli, showing that it is very promising to build a ultra-sensitive, effective, promising phototaxis design.
Reference
- 1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
- 2. Anat Bren,Martin Welch,Yuval Blat,Michael Eisenbeach.(1996) Signal termination in bacterial chemotaxis: CheZ mediates dephosphorylation of free rather than switch-bound CheY. Proc. Natl. Acad. Sci. USA, 93: 10090: 10093
- 3. Anat Bren and Micheal Eisenbeach(2000) How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation. J. Bacteriol., 182: 6865: 6873
- 4. M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280
- 5. Chenli Liu, Xiongfei Fu, Lizhong Liu,Xiaojing Ren,Carlos K.L. Chau,Sihong Li,Lu Xiang,Hualing Zeng,Guanhua Chen,Lei-Han Tang,Peter Lenz,Xiaodong Cui,Wei Huang,Terence Hwa,Jian-Dong Huang(2012) Sequential Establishment of Stripe Patterns in an Expanding Cell Population. Science, 334: 238:
- 6. Sang Ho Lee, Susan M. Butler, and Andrew Camilli(2001).Selection for in vivo regulators of bacterial virulence. Proc. Natl. Acad. Sci. USA 98: 6889: 6894
- 7. Joy sinha, Samuel J reyes, Justin p gallivan(2010).Reprogramming bacteria to seek and destroy an herbicide. Nat. Chem. Biol., 464:468
- 8. Topp, S., and Gallivan. J.P.(2008)Random Walks to Synthetic Riboswitches(2008)—A High-Throughput Selection Based on Cell Motility. Chem. Biol., 9:210:213