Team:British Columbia/Protocols

From 2012.igem.org

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{{Template:Team:British_Columbia_Header}}
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* [[Team:British Columbia/Protocols/Competent Cell Production | Competent Cell Production]] for electroporation
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<html>
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* [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry
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<style>
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* [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs
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#break {width:950px;float:left; background-color: white; margin-left: 8px;  margin-top:10px;}
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* [[Team:British Columbia/Protocols/Site Directed Mutagenesis | Site Directed Mutagenesis]]
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</style>
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* [[Team:British Columbia/Protocols/Restriction Digests | Restriction Digests]]
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* [[Team:British Columbia/Protocols/Ligations | Ligations]]
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* [[Team:British Columbia/Protocols/Electroporation | Electroporation]]
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* [https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit Mike's knockout list]
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<a href="https://2012.igem.org/Team:British_Columbia/Protocols/Competent_Cell_Production">Competent Cell Production for electroporation</a></br></br>
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* [https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE Primer Sequences]
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<a href="http://partsregistry.org/Help:Protocols/Competent_Cells">Competent Cell Production from the Parts Registry</a></br></br>
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* [https://docs.google.com/document/d/1jPujIzrW4ceKYuiwoSkoFP2maOWfh26uawRKiCX75GQ/edit Isolation of Genomic DNA from Gram-positive Bacteria]
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<a href="http://www.neb.com/nebecomm/products/protocol631.asp">Polymerase Chain Reaction (PCR) from New England BioLabs</a></br></br>
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<a href="https://2012.igem.org/Team:British_Columbia/Protocols/Site_Directed_Mutagenesis">Team:British Columbia/Protocols/Site Directed Mutagenesis">Site Directed Mutagenesis</a></br></br>
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<a href="https://2012.igem.org/Team:British_Columbia/Protocols/Restriction_Digests">Restriction Digests</a></br></br>
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<a href="https://2012.igem.org/Team:British_Columbia/Protocols/Ligations">Ligations</a></br></br>
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<a href="https://2012.igem.org/Team:British_Columbia/Protocols/Electroporation">Electroporation</a></br></br>
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<a href="https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit">Mike's knockout list</a></br></br>
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<a href="https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE">Primer Sequences</a></br></br>
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<a href="https://docs.google.com/document/d/1jPujIzrW4ceKYuiwoSkoFP2maOWfh26uawRKiCX75GQ/edit">Isolation of Genomic DNA from Gram-positive Bacteria</a></br></br>

Revision as of 07:29, 26 September 2012

British Columbia - 2012.igem.org

Competent Cell Production for electroporation

Competent Cell Production from the Parts Registry

Polymerase Chain Reaction (PCR) from New England BioLabs

Team:British Columbia/Protocols/Site Directed Mutagenesis">Site Directed Mutagenesis

Restriction Digests

Ligations

Electroporation

Mike's knockout list

Primer Sequences

Isolation of Genomic DNA from Gram-positive Bacteria