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Team:British Columbia/Protocols
From 2012.igem.org
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| - | * [[Team:British Columbia/Protocols/Competent Cell Production | Competent Cell Production]] | + | * [[Team:British Columbia/Protocols/Competent Cell Production | Competent Cell Production]] for electroporation |
* [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry | * [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry | ||
* [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs | * [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs | ||
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* [https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit Mike's knockout list] | * [https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit Mike's knockout list] | ||
* [https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE Primer Sequences] | * [https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE Primer Sequences] | ||
| + | * [https://docs.google.com/document/d/1jPujIzrW4ceKYuiwoSkoFP2maOWfh26uawRKiCX75GQ/edit Isolation of Genomic DNA from Gram-positive Bacteria] | ||
Revision as of 03:26, 26 June 2012
- Competent Cell Production for electroporation
- [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry
- [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs
- Site Directed Mutagenesis
- Restriction Digests
- Ligations
- Electroporation
