Team:LMU-Munich/Germination Stop

From 2012.igem.org

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<p align="justify">As a backup plan to make our <b>Sporo</b>beads even safer, we developed the <b>Suicide</b>switch. In case the spores do germinate, due to degradation or destruction of their outer coats, e.g. by high pressure, the <b>Suicide</b>switch will be turned on.<br></p>  
<p align="justify">As a backup plan to make our <b>Sporo</b>beads even safer, we developed the <b>Suicide</b>switch. In case the spores do germinate, due to degradation or destruction of their outer coats, e.g. by high pressure, the <b>Suicide</b>switch will be turned on.<br></p>  
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<p align="justify">It is composed by an alternative sigma factor [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823043 ECF41] ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ Wecke T., Mascher T. 2012]), which derives from ''B. lincheniformes'' and we chose to work with the short version constitutively on. It is synthetically linked to a sigma G regulated promotor responding quite late to sigma G  ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB] ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 Steil L., Völker U. et al. 2005]) responding strongly or [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823042 PsspK] ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 Steil L., Völker U. et al. 2005]) responding weakly) which is the last sigma factor activated in the forespore. Through this ecf41 is produced quite late in the forespore. Ecf41 then activates the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823041 PydfG] ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ Wecke T., Mascher T. 2012]) promotor, which is the naturally responding promoter to ECF41, which then activates the transcription of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823044 MazF] ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 Engelberg-Kulka H.,Amitai S. 2005]), a bacterial toxin from ''E.coli'' degrading mRNA. <br></p>
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<p align="justify">It is composed by an alternative sigma factor [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823043 ECF41] ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ Wecke T., Mascher T. 2012]), which derives from ''B. lincheniformes'' and we chose to work with the short version constitutively on. It is synthetically linked to a sigma G regulated promotor responding quite late to sigma G  ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB] ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 Steil L., Völker U. et al. 2005]) responding strongly or [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823042 PsspK] ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 Steil L., Völker U. et al. 2005]) responding weakly) which is the last sigma factor activated in the forespore. Through this ecf41 is produced quite late in the forespore. Ecf41 then activates the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823041 PydfG] ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ Wecke T., Mascher T. 2012]) promotor, which is the naturally responding promoter to ECF41, which then activates the transcription of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823044 MazF] ([http://jcs.biologists.org/content/118/19/4327.abstract Engelberg-Kulka H.,Amitai S. 2005]), a bacterial toxin from ''E.coli'' degrading mRNA. <br></p>
<p align="justify">The idea behind this is to pack the <b>Sporo</b>beads full with ECF41 when they sporulate, which will in turn kill them upon germination due to the MazF. <br></p>
<p align="justify">The idea behind this is to pack the <b>Sporo</b>beads full with ECF41 when they sporulate, which will in turn kill them upon germination due to the MazF. <br></p>
<p align="justify">Through the system with the alternative sigma factor the forespore gains hopefully enough time to fully mature before MazF starts to be produced.<br></p>
<p align="justify">Through the system with the alternative sigma factor the forespore gains hopefully enough time to fully mature before MazF starts to be produced.<br></p>

Revision as of 07:42, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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