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Team:Paris-Saclay/Project/Notebook/Week 10
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| - | | style="width: 50%;"| | + | | style="width: 50%;"|New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. |
| style="width: 35%;"| [[File:Week10-4.jpg|right|330px]] | | style="width: 35%;"| [[File:Week10-4.jpg|right|330px]] | ||
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*Digestion by EcoRI of the plasmid that contains BBa_K274100. | *Digestion by EcoRI of the plasmid that contains BBa_K274100. | ||
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| + | ====10th August==== | ||
| + | |||
| + | {| width="85%" | ||
| + | |- | ||
| + | | style="width: 50%;"|Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel. | ||
| + | | style="width: 35%;"| [[File:Week10-5.jpg|right|330px]] | ||
| + | |} | ||
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| + | PCR program used: | ||
| + | [[File:Week10-6.jpg|600px]] | ||
Revision as of 20:50, 25 September 2012
Contents |
6th August
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th August
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th August
| Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid. |  |
PCR program used:
9th August
| New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. |  |
- Digestion by EcoRI of the plasmid that contains BBa_K274100.
10th August
| Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel. |  |
PCR program used:
