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Team:SDU-Denmark/labwork/Notebook/week4
From 2012.igem.org
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We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on. <br/> | We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on. <br/> | ||
We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector. | We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector. | ||
| - | These will be shipped of to be sequneced | + | These will be shipped of to be sequneced. |
<br/> <br/> | <br/> <br/> | ||
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<p> | <p> | ||
| - | The two sample from | + | The two sample from earlier were prepared for sequencing by diluting them to 70-78 ng/μl and then send. Afterwards we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing. |
Revision as of 08:15, 26 September 2012
Laboratory Notebook
23-07-2012 to 29-07-2012
SST send for sequencing
SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.
Cryo backup, miniprep and preparation to sequencing
We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector.
These will be shipped of to be sequneced.
SST send for sequencing
The two sample from earlier were prepared for sequencing by diluting them to 70-78 ng/μl and then send. Afterwards we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing.
