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Team:LMU-Munich/Results
From 2012.igem.org
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===Germination Gene Knockouts=== | ===Germination Gene Knockouts=== | ||
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| + | {| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;" | ||
| + | | style="width: 70%;background-color: #EBFCE4;" | | ||
| + | {|align:center | ||
| + | |[[File:Germination_RatesII.jpg|600px|center]] | ||
| + | |- | ||
| + | | style="width: 80%;background-color: #EBFCE4;" | | ||
| + | {| style="color:black;" cellpadding="3" width="95%" cellspacing="0" border="0" align="left" style="text-align:left;" | ||
| + | |style="width: 70%;background-color: #EBFCE4;" | | ||
| + | <font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (WT168), our positive control. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Mutants used were: <br /> | ||
| + | '''WT168''': Wild-type 168 <br /> | ||
| + | '''''spo0A''::tet''': A sporulation-deficient strain <br /> | ||
| + | '''B29''': ''cwlD''::kan, ''sleB''::mls <br /> | ||
| + | '''B30''': ''gerD''::cm, ''sleB''::mls <br /> | ||
| + | '''B32''': ''cwlJ''::spec, ''cwlD''::kan <br /> | ||
| + | '''B40''': ''cwlD''::kan, ''sleB''::mls, ''cwlJ''::spec <br /> | ||
| + | '''B41''': ''cwlD''::kan, ''sleB''::mls, ''gerD''::cm <br /> | ||
| + | '''B42''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm <br /> | ||
| + | '''B43''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec <br /> | ||
| + | '''B46''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm, ''sleB''::mls <br /> | ||
| + | '''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan</font> | ||
| + | |} | ||
| + | |} | ||
| + | |} | ||
Revision as of 20:54, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Here you will find the main results of our work.
B. subtilis vectors
| Name BioBrick | E. coli res. | B. subt. res. | Insertion locus | Description | Cloning status | Works? |
|---|---|---|---|---|---|---|
| pSBBs1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 (BBa_K823023)] | Amp | Cm | amyE | empty |  | |
| pSBBs4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 (BBa_K823022)] | Amp | Spec | thrC | empty | | |
| pSBBs2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 (BBa_K823027)] | Amp | MLS | lacA | empty | in work | not tested |
| pSBBs1C-lacZ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 (BBa_K823021) ] | Amp | Cm | amyE | lacZ reporter | | |
| pSBBs3C-luxABCDE [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 (BBa_K823025)] | Amp | Cm | sacA | luxABCDE reporter | | |
| pSBBs4S-PXyl [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 (BBa_K823024)] | Amp | Spec | thrC | Xylose-promoter | | refuses transformation |
| pSBBs0K-Pspac [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823026)] | Amp | Kan | replicative | IPTG-promoter | | unexpected |
| Sporovector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823054)] | Amp | Spec | thrC | to make Sporobeads | | not tested |
Inverter
Germination Gene Knockouts
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For detailed results visit our data page and for all background info our project page.
