Team:WashU/Week4
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The team has continued to attempt to biobrick together the necessary genes that create the carotenoid pathway in E. coli (see last week for more information). To do this, the team followed the biobrick assembly protocol once more, synthesizing the upstream, downstream and plasmid parts, and then connected them. In order to increase the efficiency and yield of this assembly, gel purification was used to isolate the plasmid for the biobrick assembly. Below is the gel with the upstream, downstream and plasmid parts. <br> | The team has continued to attempt to biobrick together the necessary genes that create the carotenoid pathway in E. coli (see last week for more information). To do this, the team followed the biobrick assembly protocol once more, synthesizing the upstream, downstream and plasmid parts, and then connected them. In order to increase the efficiency and yield of this assembly, gel purification was used to isolate the plasmid for the biobrick assembly. Below is the gel with the upstream, downstream and plasmid parts. <br> | ||
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==Modeling== | ==Modeling== | ||
WashUiGEM has continued to develop two different models - a small scale model and a genome-scale model - using MATLAB. | WashUiGEM has continued to develop two different models - a small scale model and a genome-scale model - using MATLAB. |
Revision as of 21:30, 25 June 2012