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Team:Bielefeld-Germany/Labjournal
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*Start of our WET LAB time. | *Start of our WET LAB time. | ||
* Generating new competent E.coli cells. | * Generating new competent E.coli cells. | ||
| - | * | + | * Successful PCRs of laccase genes CopA from ''Xanthomonas campestris B100'', CueO from ''E. coli BL21(DE3)'' and CotA from ''Bacillus pumilus ATCC7061'' with genomic DNA as template. The primers were designed with T7 promotor-overhanging ends after prefix and HIS-Tag before suffix. |
==Week 2 (07.05.- 13.05.12) == | ==Week 2 (07.05.- 13.05.12) == | ||
Revision as of 12:27, 27 June 2012
Contents |
Week 1 (30.04.-06.05.12)
- Start of our WET LAB time.
- Generating new competent E.coli cells.
- Successful PCRs of laccase genes CopA from Xanthomonas campestris B100, CueO from E. coli BL21(DE3) and CotA from Bacillus pumilus ATCC7061 with genomic DNA as template. The primers were designed with T7 promotor-overhanging ends after prefix and HIS-Tag before suffix.
Week 2 (07.05.- 13.05.12)
Doing the First Steps to prepare a the Student Acadamy in cooperation with the CeBiTec. Finding suitable BioBricks to develop an coulourful and easy experiment. Trying to isolate the GFP and RFP containing plasmid and to transform an Plasmidmix to produce colored Agar-plates. Isolation of three Laccase genes und to amplify the genetic sequences ... SUCESS Next step..... integration of the sequences in the Backbone pSB1C3.
