Team:XMU-China/datapage

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Logic gate
Green Fluorescent Protein
Cell immobilization
Quorum Sensing
Time delay
Original Design
Project Overview
References

XMU-DATAPAGE

Data Page

The Design



Figure 1: Digital display could output number or letter when input a specific signal. It also can accept different signals to output more information when structure gene circuits under logical gates system.



Figure 2:After the induction by arabinose, the duration of response time for GFP expression increases as the strength of RBS declines, bringing about a time-course display.

Data From New Parts Submitted To The Registry

Parts Of Binary System

BBa_K750000, BBa_K750103, BBa_K750111 - these parts contain P_BAD (BBa_K206000), P_cI (BBa_R0051) and P_tet (BBa_J102002) respectively and they both contain gfp-lva (BBa_K145015). We have characterized the expression of these parts on Digital Display section of our project in the wiki.

Parts Of Ternary System

BBa_K750102, BBa_K750104, BBa_K750105, BBa_K750106, BBa_K750107 - these parts both contain cI (BBa_C0051) and are used to control parts promoted by P_cI (BBa_R0051). We also have characterized the expression of these parts by protein electrophoresis on Digital Display section of our project in the wiki.

BBa_K750108, BBa_K750109, BBa_K750110, BBa_K750112, BBa_K750113, BBa_K750114, BBa_K750115 – these composite parts are made up of BBa_K750000, BBa_K750102, BBa_K750103, BBa_K750104 and BBa_K750111. Most of them have been characterized on Digital Display section of our project in the wiki.

Parts of Time Delay System

BBa_K750001, BBa_K750002, BBa_K750003, BBa_K750004, BBa_K750005 - these parts contain RBS_1.0 (BBa_B0034), RBS_0.6 (BBa_B0030), RBS_0.3 (BBa_B0032), RBS_0.07 (BBa_B0031) and RBS_0.01 (BBa_B0033) respectively and they both contain luxI (BBa_C0061). These parts, promoted by arabinose, are used to control the expression of AHL.

BBa_K750006 – this part contains luxR (BBa_C0062) and is promoted by arabinose.

BBa_K750009, BBa_K750010, BBa_K750011, BBa_K750012 – these composite parts are characterized on Time Delay section of our project in the wiki.

Data From Characterized Parts Already In The Registry

BBa_K145015 – characterized the gene gfp-lva by protein electrophoresis. Please see our Digital Display section for more information.

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-     <td colspan="2" id="bor"><b>Figure 1:</b> Digital display could output number or letter when input  a specific  signal. It also can accept different  signals to output more information.</td></tr>
+     <td colspan="2" id="bor"><b>Figure 1:</b> Digital display could output number or letter when input  a specific  signal. It also can accept different  signals to output more information when structure gene circuits under logical gates system.</td></tr>
 </table>
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 <p><strong class="subtitle"><a name="_Toc02"></a>Data From New Parts Submitted To The Registry</strong><br><br>
 <strong class="sub3title">Parts Of Binary System</strong><br><br>
-<li><b>BBa_K750000</b>, <b>BBa_K750103</b>, <b>BBa_K750111</b> - these parts contain <i>P<sub>BAD</sub></i> (BBa_K206000), <i>P<sub>cI</sub></i> (BBa_R0051) and <i>P<sub>tet</sub></i> (BBa_J102002) respectively and they both contain <i>gfp-lva</i> (BBa_K145015). We have characterized the expression of these parts on Digital Display section of our project in the wiki.</li><br>
+<li><b>BBa_K750000</b>, <b>BBa_K750103</b>, <b>BBa_K750111</b> - these parts contain <i>P<sub>BAD</sub></i> (BBa_K206000), <i>P<sub>cI</sub></i> (BBa_R0051) and <i>P<sub>tet</sub></i> (BBa_J102002) respectively and they both contain <i>gfp-lva</i> (BBa_K145015). We have characterized the expression of these parts on <a href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">Digital Display</a> section of our project in the wiki.</li><br>
 <strong class="sub3title">Parts Of Ternary System</strong><br><br>
-<li><b>BBa_K750102</b>, <b>BBa_K750104</b>, <b>BBa_K750105</b>, <b>BBa_K750106</b>, <b>BBa_K750107</b> - these parts both contain <i>cI</i> (BBa_C0051). they are used to regulate parts controled by <i>P<sub>cI</sub></i> (BBa_R0051). We also have characterized the expression of these parts by protein electrophoresis on Digital Display section of our project in the wiki.</li>
+<li><b>BBa_K750102</b>, <b>BBa_K750104</b>, <b>BBa_K750105</b>, <b>BBa_K750106</b>, <b>BBa_K750107</b> - these parts both contain <i>cI</i> (BBa_C0051) and are used to control parts promoted by <i>P<sub>cI</sub></i> (BBa_R0051). We also have characterized the expression of these parts by protein electrophoresis on <a href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">Digital Display</a> section of our project in the wiki.</li>
-<li><b>BBa_K750108</b>, <b>BBa_K750109</b>, <b>BBa_K750110</b>, <b>BBa_K750112</b>, <b>BBa_K750113</b>, <b>BBa_K750114</b>, <b>BBa_K750115</b> – these composite parts are made up of BBa_K750000, BBa_K750102, BBa_K750103, BBa_K750104 and BBa_K750111. Most of them have been characterized on Digital Display section of our project in the wiki.</li><br>
+<li><b>BBa_K750108</b>, <b>BBa_K750109</b>, <b>BBa_K750110</b>, <b>BBa_K750112</b>, <b>BBa_K750113</b>, <b>BBa_K750114</b>, <b>BBa_K750115</b> – these composite parts are made up of BBa_K750000, BBa_K750102, BBa_K750103, BBa_K750104 and BBa_K750111. Most of them have been characterized on <a href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">Digital Display</a> section of our project in the wiki.</li><br>
 <strong class="sub3title">Parts of Time Delay System</strong><br><br>
 <li><b>BBa_K750001</b>, <b>BBa_K750002</b>, <b>BBa_K750003</b>, <b>BBa_K750004</b>, <b>BBa_K750005</b> - these parts contain
-<i>RBS<sub>1.0</sub></i> (BBa_B0034), <i>RBS<sub>0.6</sub></i> (BBa_B0030), <i>RBS<sub>0.3</sub></i> (BBa_B0032), <i>RBS<sub>0.07</sub></i> (BBa_B0031) and <i>RBS<sub>0.01</sub></i> (BBa_B0033) respectively and they both contain <i>luxI</i> (BBa_C0061). These parts contain <i>P<sub>BAD</sub></i> (BBa_K206000), induced by arabinose, are used to control the expression of AHL.</li>
+<i>RBS<sub>1.0</sub></i> (BBa_B0034), <i>RBS<sub>0.6</sub></i> (BBa_B0030), <i>RBS<sub>0.3</sub></i> (BBa_B0032), <i>RBS<sub>0.07</sub></i> (BBa_B0031) and <i>RBS<sub>0.01</sub></i> (BBa_B0033) respectively and they both contain <i>luxI</i> (BBa_C0061). These parts, promoted by arabinose, are used to control the expression of AHL.</li>
 <li><b>BBa_K750006</b> – this part contains <i>luxR</i> (BBa_C0062) and is promoted by arabinose.</li>
-<li><b>BBa_K750009, BBa_K750010, BBa_K750011, BBa_K750012</b> – these composite parts are characterized on Digital Display section of our project in the wiki.</li>
+<li><b>BBa_K750009, BBa_K750010, BBa_K750011, BBa_K750012</b> – these composite parts are characterized on <a href="https://2012.igem.org/Template:Team:XMU-China/timedelay">Time Delay</a> section of our project in the wiki.</li>
 </p>
 <hr>
   <p><strong class="subtitle"><a name="_Toc03"></a>Data From Characterized Parts Already In The Registry</strong><br>
-  <li><b>BBa_K145015</b> – characterized the gene <i>gfp-lva</i> by protein electrophoresis. Please see our Time Delay section for more information.</li><br>
+  <li><b>BBa_K145015</b> – characterized the gene <i>gfp-lva</i> by protein electrophoresis. Please see our <a href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">Digital Display</a> section for more information.</li><br>
 </p>