Team:Freiburg/Notebook/TAL

From 2012.igem.org

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{{Template:Team:Freiburg}}
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__NOTOC__
=<span style="color:#2244AA"> Our way to the TAL vector =
=<span style="color:#2244AA"> Our way to the TAL vector =
 +
----
 +
<br>
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==08/08/12==
 +
1.) MutPCR for pSB1C3 vector backbone in order to remove restriction sites vor BsmBI<br>
 +
2.) Transformation of Sirt2 and HAT5<br>
 +
3.) GGC in order to produce our MammoBrick
 +
1.) MutPCR: 
-
//
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<table border="0" style="background-color:transparent">
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<tr>
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<th></th>
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<th>Tube 1 [µl]</th>
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<th>Tube 2 [µl]</th>
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</tr>
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<tr>
 +
<td>Primer MutPCR fo [10 mM]</td>
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<td>1</td><td>0,25</td>
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</tr>
 +
<tr>
 +
<td>Primer MutPCR re [10 mM]</td>
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<td>1</td><td>0,25</td>
 +
</tr>
 +
<tr>
 +
<td>dNTPs [10 mM]</td>
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<td>1</td><td>1</td>
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</tr>
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<tr>
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<td>Phusion Buffer HF</td>
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<td>1,25</td><td>1,25</td>
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</tr>
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<tr>
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<td>Phusion Polymerase</td>
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<td>0,25</td><td>0,25</td>
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</tr>
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<tr>
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<td>DMSO</td>
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<td>1</td><td>1</td>
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</tr>
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<tr>
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<td>MgCl2</td>
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<td>1</td><td>1</td>
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</tr>
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<tr>
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<td>Template</td>
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<td>1</td><td>1</td>
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</tr>
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<tr>
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<td>ddH2O</td>
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<td>5</td><td>6,5</td>
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</tr>
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</table>

Revision as of 16:27, 25 September 2012








Our way to the TAL vector


08/08/12

1.) MutPCR for pSB1C3 vector backbone in order to remove restriction sites vor BsmBI
2.) Transformation of Sirt2 and HAT5
3.) GGC in order to produce our MammoBrick

1.) MutPCR:

Tube 1 [µl] Tube 2 [µl]
Primer MutPCR fo [10 mM] 10,25
Primer MutPCR re [10 mM] 10,25
dNTPs [10 mM] 11
Phusion Buffer HF 1,251,25
Phusion Polymerase 0,250,25
DMSO 11
MgCl2 11
Template 11
ddH2O 56,5
Retrieved from "http://2012.igem.org/Team:Freiburg/Notebook/TAL"