Team:LMU-Munich/Bacillus BioBricks/integration

From 2012.igem.org

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==Integrative vectors for ''Bacillus subtilis''==
==Integrative vectors for ''Bacillus subtilis''==
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<p align="justify">
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Many vectors for ''B. subtilis'' are integrative, so are all except for one that we provide to the registry. They all insert into the genome via double homologous recombination at specific sites. A target gene in ''B. subtilis'' is disrupted which can be selected for by specific tests. These are part of our protocol [https://static.igem.org/mediawiki/2012/c/c4/LMU-Munich_2012_Bacillus_subtilis_vectors.pdf "How to use ''Bacillus subtilis'' vectors"].
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All except for one vector that we provided to the registry for ''B. subtilis'' are integrative. They all insert into the genome via double homologous recombination at specific sites. Thereby, a target gene in ''B. subtilis'' is disrupted, which can be selected for by specific tests. The tests are part of our protocol [https://static.igem.org/mediawiki/2012/c/c4/LMU-Munich_2012_Bacillus_subtilis_vectors.pdf "How to use ''Bacillus subtilis'' vectors"].
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The following grafic shows how the integration works.
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
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|[[File:LMU-Munich-integration-Picture1.png|500px|center]]
|[[File:LMU-Munich-integration-Picture1.png|500px|center]]
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|'''A''': ''Bacillus'' vector
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|'''A''': ''Bacillus subtilis'' vector
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|[[File:LMU-Munich-integration-Arrow.png|80px|center]]
|[[File:LMU-Munich-integration-Arrow.png|80px|center]]

Latest revision as of 16:28, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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