Team:TU Munich/Notebook/Labjournal

From 2012.igem.org

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(→Quick Change mutagenis to remove NgoMIV from pYES2)
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|4 µl
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|P7 template
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|Plasmid P7 pYes2_RFC25 MCS template
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|0.5 µl
|0.5 µl

Revision as of 15:29, 23 June 2012

Saturday, 23th June

Quick Change mutagenis to remove NgoMIV from pYES2

Investigator: Ingmar, Volker


PCR reaction batch

volume substance
2.5 µl 10x Pfu Ultra II buffer
4 µl Plasmid P7 pYes2_RFC25 MCS template
0.5 µl 1:10 dilution of O38 (10 pmol/µL)
0.5 µl 1:10 dilution of O39 ((10 pmol/µL)
17 µl ddH2O
0.5 µl dNTP mix
0.5 µl Pfu Turbo DNA polymerase (2.5 U / µl)
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