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Team:LMU-Munich/Bacillus BioBricks/integration
From 2012.igem.org
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==Integrative vectors for ''Bacillus subtilis''== | ==Integrative vectors for ''Bacillus subtilis''== | ||
| - | Many vectors for ''B. subtilis'' are integrative, so are all except for one that we provide to the registry. They all insert into the genome via double homologous recombination at specific sites. A target gene in ''B. subtilis'' is disrupted which can be selected for by specific tests. These are part of our protocol [https://static.igem.org/mediawiki/2012/c/c4/LMU-Munich_2012_Bacillus_subtilis_vectors.pdf | + | Many vectors for ''B. subtilis'' are integrative, so are all except for one that we provide to the registry. They all insert into the genome via double homologous recombination at specific sites. A target gene in ''B. subtilis'' is disrupted which can be selected for by specific tests. These are part of our protocol [https://static.igem.org/mediawiki/2012/c/c4/LMU-Munich_2012_Bacillus_subtilis_vectors.pdf "How to use ''Bacillus subtilis'' vectors"]. |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;" | {| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;" | ||
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| - | <font color="#000000"; size="2"> | + | <font color="#000000"; size="2">Integration of ''B. subtilis'' vector into the genome.</font> |
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| - | + | *'''A''': ''Bacillus'' vector | |
| + | *'''B''': Linearisation with ''Sca''I | ||
| + | *'''C''': Linearized vector is transfor | ||
Revision as of 10:08, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Integrative vectors for Bacillus subtilis
Many vectors for B. subtilis are integrative, so are all except for one that we provide to the registry. They all insert into the genome via double homologous recombination at specific sites. A target gene in B. subtilis is disrupted which can be selected for by specific tests. These are part of our protocol "How to use Bacillus subtilis vectors".
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- A: Bacillus vector
- B: Linearisation with ScaI
- C: Linearized vector is transfor
