Team:Exeter/Achievements

From 2012.igem.org

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     <ul>
     <ul>
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned and submitted 14 biobricks into the registry</li>
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned and submitted 14 biobricks into the registry</li>
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Obtained <i>wbbC</i> gene from BL21 genome</li>
+
<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned over 32 more Biobricks waiting to be transferred into pSB1C3 plasmids</li>
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoBase; a database containing a list of >100 glycosyltransferase enzymes  
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Using PCR we obtained <i>wbbC</i> gene from BL21 genome</li>
-
     from <i>E.coli</i> strains to demonstrate the vast linkages we could achieve only with <i>E.coli</i> enzymes</li>
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoBase; a database containing a list of over 100 different glycosyltransferase enzymes mainly
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoWeb, an interface for an online user to access Glycobase with a
+
     from <i>E.coli</i> strains demonstrating the vast linkages we could achieve with just <i>E.coli</i> enzymes</li>
-
    polysaccharide in mind and the database searches and finds the enzymes you’d use to make it.</li>
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoWeb, an interface for an online user to access Glycobase. Glycoweb has the ability to tell you which enzymes are needed to create your bespoke polysaccharide.</li>
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoApp; an application for a handheld device to access the Glycobase from
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoApp; an application for a handheld device to access the Glycobase giving the user distance access and enhanced ordering capabilities</li>
-
    wherever the consumer is when they realise they need a polysaccharide ordering!</li>
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Considered human practice approaches and discussed with several experts the human  
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Considered human practice approaches and discussed with several experts the human  
     practice considerations, hosted a human practice panel and discussed with the wider community at Café Scientifique.</li>
     practice considerations, hosted a human practice panel and discussed with the wider community at Café Scientifique.</li>
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Discussed our project with various businesses including Shell, Ginsters etc. and we  
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Discussed our project with various businesses including Shell and we  
-
     received letters of support from DSTL, ISCA Biochemical Ltd, Avon. </li>
+
     received letters of support from DSTL, ISCA Biochemical Ltd and Avon. </li>
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Demonstrated that….. Successfully expressed this polysaccharide….. (to be cont….)</li>
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Demonstrated that….. Successfully expressed this polysaccharide….. (to be cont….)</li>
-
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Made promoter-RBS-gene-terminator constructs for each gene</li>
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Made promoter-RBS-gene-terminator constructs for two of the genes with more in construction</li>
 +
<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">We managed to obtain 7 of 9 fragments for Gibson assembly through PCR. The different annealing temperatures of the primers made this hard but showed how important the preparation for Gibson has to be</li>
 +
<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">In touching distance of completing our operons. Only time stopped this happening.</li>
 +
<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">We were one construct away from being able to complete our 3 gene inducible plasmid.</li>
 +
 
     </ul>
     </ul>
     <br>
     <br>
     <br>
     <br>
-
     <ul>
+
     <ul>  
-
    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">A correct mass analysis of the product is essential, but cannot alone confirm the
+
-
    correct product. The possibility that an incorrect product has been made through incorrect synthesis or post-synthetic regiospecific or enantiospecific rearrangement by
+
-
    chemical or enzymatic means must be examined and ruled out before the method can be considered reliable.</li>
+
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Synthesis of a previously well characterised small polysaccharide, which can be  
     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Synthesis of a previously well characterised small polysaccharide, which can be  
     physically and also biochemically characterised, to show that the product will act in biological systems as expected. Once a fully characterised polysaccharide is produced, we  
     physically and also biochemically characterised, to show that the product will act in biological systems as expected. Once a fully characterised polysaccharide is produced, we  
     would be in a much stronger position to offer novel products and to start to address the exciting possibilities of our method.</li>
     would be in a much stronger position to offer novel products and to start to address the exciting possibilities of our method.</li>
-
    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Only managed to obtain 7 of 9 fragments for Gibson assembly through PCR.</li>
 
-
    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Didn’t manage to construct operons through BioBrick assembly.</li>
 
     </ul>
     </ul>
   </font>
   </font>

Revision as of 14:43, 26 September 2012

Protocol 6

Achievements and Failures
  • Successfully cloned and submitted 14 biobricks into the registry
  • Successfully cloned over 32 more Biobricks waiting to be transferred into pSB1C3 plasmids
  • Using PCR we obtained wbbC gene from BL21 genome
  • Created GlycoBase; a database containing a list of over 100 different glycosyltransferase enzymes mainly from E.coli strains demonstrating the vast linkages we could achieve with just E.coli enzymes
  • Created GlycoWeb, an interface for an online user to access Glycobase. Glycoweb has the ability to tell you which enzymes are needed to create your bespoke polysaccharide.
  • Created GlycoApp; an application for a handheld device to access the Glycobase giving the user distance access and enhanced ordering capabilities
  • Considered human practice approaches and discussed with several experts the human practice considerations, hosted a human practice panel and discussed with the wider community at Café Scientifique.
  • Discussed our project with various businesses including Shell and we received letters of support from DSTL, ISCA Biochemical Ltd and Avon.
  • Demonstrated that….. Successfully expressed this polysaccharide….. (to be cont….)
  • Made promoter-RBS-gene-terminator constructs for two of the genes with more in construction
  • We managed to obtain 7 of 9 fragments for Gibson assembly through PCR. The different annealing temperatures of the primers made this hard but showed how important the preparation for Gibson has to be
  • In touching distance of completing our operons. Only time stopped this happening.
  • We were one construct away from being able to complete our 3 gene inducible plasmid.


  • Synthesis of a previously well characterised small polysaccharide, which can be physically and also biochemically characterised, to show that the product will act in biological systems as expected. Once a fully characterised polysaccharide is produced, we would be in a much stronger position to offer novel products and to start to address the exciting possibilities of our method.

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