Team:LMU-Munich/Bacillus Introduction
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Revision as of 08:31, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Bacillus subtilis - a new chassis for iGEM
Here we summarize some aspects of Bacillus subtilis that we think are important to mention
Organism | B. subtilis | E. coli |
---|---|---|
type | gram positive rod | gram negative rod |
Motility | pos | pos |
Safety | GRAS | GRAS |
Transformation | natural competence | artificial competence |
Vectors | integrative and replicative | replicative |
Sporulation | pos | pos |
Differentiation | pos | pos |
Visit our Protocols page for details how to work with B. subtilis
Mostly E. coli is used as the general chassis in the iGEM competition. We are using B. subtilis as chassis for a few reasons. There are two major differences between B. subtilis and E. coli that are of interest to us:
1. B. subtilis is able to differentiate into cells with different morphology and function, the most severe form being the endospore which is formed under stress conditions. But there are also phenomena like cannibalism which makes B. subtilis a lot more diverse to work with. We will exploit the production of endospores in our project Beadzillus. The life cycle of B. subtilis is depicted in Figure 1.
2. B. subtilis can replicate exogenic DNA via an origin of replication on a plasmid as E. coli does, but there is a much more elegant way of bringing in exogenic DNA stretches. When flanked by homologous regions to the bacterial genome, it will integrate at high efficiency via homologous recombination at this locus and furthermore be replicated with the genome. This has the advantage that if comparing different things, not only the enviroment is always the same, but also the copy number is from cell to cell and from strain to strain the same, which is not always the case for replicative plasmids. This integrative way of bringing in exogenic DNA will be exploited by us when producing the BioBrick compatible Bacillus vectors. The comparision between these two ways of bringing in exogenic DNA is depicted in Figure 2.
For these reasons in some cases B. subtilis can be the chassis of choice. Sadly very few teams have worked with this model organism and there is at this time no established BioBrick system to use B. subtilis as a chassis.
Bacillus Intro | Bacillus BioBrickBox | Sporobeads | Germination STOP |