Team:LMU-Munich/Germination Stop

From 2012.igem.org

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<p align="justify">As a backup plan to make our <b>Sporo</b>beads even safer, we developed the <b>Suicide</b>switch. In case the spores do germinate, due to degradation or destruction of their outer coats, e.g. by high pressure, the <b>Suicide</b>switch will be turned on.<br></p>  
<p align="justify">As a backup plan to make our <b>Sporo</b>beads even safer, we developed the <b>Suicide</b>switch. In case the spores do germinate, due to degradation or destruction of their outer coats, e.g. by high pressure, the <b>Suicide</b>switch will be turned on.<br></p>  
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<p align="justify">It is composed by an alternative sigma factor [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ ecf41], which derives from ''B. lincheniformes'', which is synthetically linked to a sigma G regulated promotor responding quite late to sigma G ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 PspoIVB] responding strongly or [http://www.ncbi.nlm.nih.gov/pubmed/15699190 PsspK] responding weakly) which is the last sigma factor activated in the forespore. Ecf41 then activates the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ PydfG] promotor, which is the naturally responding promoter to ecf41, which then activates the transcription of [http://jcs.biologists.org/content/118/19/4327.abstract MazF], a bacterial toxin from ''E.coli'' degrading mRNA. <br></p>
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<p align="justify">It is composed by an alternative sigma factor [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ ecf41], which derives from ''B. lincheniformes'' and we chose to work with the short version constitutively on. It is synthetically linked to a sigma G regulated promotor responding quite late to sigma G ([http://www.ncbi.nlm.nih.gov/pubmed/15699190 PspoIVB] responding strongly or [http://www.ncbi.nlm.nih.gov/pubmed/15699190 PsspK] responding weakly) which is the last sigma factor activated in the forespore. Through this ecf41 is produced quite late in the forespore. Ecf41 then activates the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426412/ PydfG] promotor, which is the naturally responding promoter to ecf41, which then activates the transcription of [http://jcs.biologists.org/content/118/19/4327.abstract MazF], a bacterial toxin from ''E.coli'' degrading mRNA. <br></p>
<p align="justify">The idea behind this is to pack the <b>Sporo</b>beads full with ecf41 when they sporulate, which will in turn kill them upon germination due to the MazF. <br></p>
<p align="justify">The idea behind this is to pack the <b>Sporo</b>beads full with ecf41 when they sporulate, which will in turn kill them upon germination due to the MazF. <br></p>
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<p align="justify">Through the system with the alternative sigma factor the forespore gains hopefully enough time to fully mature before MazF starts to be produced.<br></p>
<p align="justify">We chose MazF, as we think it will not be able to harm our <b>Sporo</b>bead even if it is made too early, as the spore does not rely on translation to be preserved. <br></p>
<p align="justify">We chose MazF, as we think it will not be able to harm our <b>Sporo</b>bead even if it is made too early, as the spore does not rely on translation to be preserved. <br></p>
We are planning to model this system.
We are planning to model this system.

Revision as of 09:26, 25 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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