Team:British Columbia/Protocols
From 2012.igem.org
(Difference between revisions)
Line 4: | Line 4: | ||
* [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry | * [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry | ||
* [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs | * [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs | ||
+ | * [[Team:British Columbia/Protocols/Site Directed Mutagenesis | Site Directed Mutagenesis]] | ||
+ | * [[Team:British Columbia/Protocols/Restriction Digests | Restriction Digests]] | ||
+ | * [[Team:British Columbia/Protocols/Ligations | Ligations]] | ||
+ | * [[Team:British Columbia/Protocols/Electroporation | Electroporation]] | ||
+ | |||
* [https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit Mike's knockout list] | * [https://docs.google.com/document/d/1P4zsHD8Zd74sWvJVhV5A8VctAz5oEvrE8-K0gItPCvc/edit Mike's knockout list] | ||
* [https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE Primer Sequences] | * [https://docs.google.com/spreadsheet/ccc?key=0AvwvAUH-9nhjdEk5WW5yYUJLNUNqTnZRSlNvT3hhOEE Primer Sequences] | ||
- |
Revision as of 00:06, 26 June 2012
- Competent Cell Production
- [http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cell Production] from the Parts Registry
- [http://www.neb.com/nebecomm/products/protocol631.asp PCR] from New England BioLabs
- Site Directed Mutagenesis
- Restriction Digests
- Ligations
- Electroporation