Team:MIT/Results
From 2012.igem.org
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- | <b> Strategy 1: | + | <b> Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper</b> |
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- | < | + | <br> Strategy 2: Switch Transfection reagent to RNAiMAX </b> |
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+ | <b> Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker </b> | ||
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- | <b> Strategy | + | <br> |
+ | <b> Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter </b> | ||
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- | <b> | + | <b> [Strategy 5]: Redesign RNA Reporter </b> |
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Revision as of 04:50, 25 September 2012
RNA Strand Displacement In Vitro
Nucleic Acid Delivery
In Vivo RNA Strand Displacement
Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper
Strategy 2: Switch Transfection reagent to RNAiMAX
Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker
Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter
Strategy 4: Nucleofect RNA reporter, RNA inputs
[Strategy 5]: Redesign RNA Reporter
Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper
Strategy 2: Switch Transfection reagent to RNAiMAX
Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker
Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter
Strategy 4: Nucleofect RNA reporter, RNA inputs
[Strategy 5]: Redesign RNA Reporter