Team:Exeter/lab book/proto

(Difference between revisions)

Revision as of 00:53, 25 September 2012

Protocol Home


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase

Protocols
Transformation Protocol from Invitrogen, for Top10 Competent Cells Transferring Cultures to Liquid Medium Mini Prep New England BioLabs Biobrick Assembly Protocol Annealing Oligomer Primers Biobrick Extraction Two-Step PCR Bacterial Genomic DNA Extraction Using GenElute PCR purification using QIAquick Linearized Plasmid Backbone Digestion and Ligation Protocol Preparation of IDTE Buffer and IDT Re-Suspension 3-Step PCR

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      <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/9" style="color:#1d1d1b"><u>PCR purification using QIAquick</u></a></li>
-     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/10" style="color:#1d1d1b"><u>Linearized plasmid backbone digestion and ligation protocol</u></a></li>
+     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/10" style="color:#1d1d1b"><u>Linearized Plasmid Backbone Digestion and Ligation Protocol</u></a></li>
-     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE buffer and IDT re-suspension</u></a></li>
+     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE Buffer and IDT Re-Suspension</u></a></li>
-     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-step PCR</u></a></li>
+     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-Step PCR</u></a></li>
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