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Team:Potsdam Bioware/slider
From 2012.igem.org
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| + | To produce highly affine antibodies, a CHO (Chinese Hamster Ovary) cell line is transfected with a low affine antibody construct. The plasmid pcDNA5/FRT is used to allow the easy integration of the gene of interest into the CHO cell genome via FLP-In sites. This integration is catalyzed by the FLP-recombinase in the pOG44 plasmid. | ||
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Revision as of 10:03, 25 September 2012
To produce highly affine antibodies, a CHO (Chinese Hamster Ovary) cell line is transfected with a low affine antibody construct. The plasmid pcDNA5/FRT is used to allow the easy integration of the gene of interest into the CHO cell genome via FLP-In sites. This integration is catalyzed by the FLP-recombinase in the pOG44 plasmid.
