Biobricks

<partinfo>BBa_K929000 short</partinfo>

[http://partsregistry.org/Part:BBa_K929000 CMV_wtAID_pA]
BioBrick Nr.	[http://partsregistry.org/Part:BBa_929000 BBa_929000]
RFC standard	[http://partsregistry.org/Help:Assembly_standard_10 RFC 10]
Requirement	pSB1C3
Source	existing parts:([http://partsregistry.org/Part:BBa_K103001 BBa_K103001];[http://partsregistry.org/Part:BBa_I712004 BBa_I712004]; [http://partsregistry.org/Part:BBa_K404108 BBa_K404108])
Submitted by	Potsdam_Bioware2012

Figure 1:pSB1C3-Plasmid with AID with CMV promoter and hGH-polyadenylation signal sequence

The BioBrick "AID with CMV promoter and hGH-polyadenylation signal sequence" ([http://partsregistry.org/Part:BBa_K929000 BBa_K929000]) is an extended version of the existing AID BioBrick ([http://partsregistry.org/Part:BBa_K103001 BBa_K103001]). It is built of 3 parts: CMV promoter ([http://partsregistry.org/Part:BBa_I712004 BBa_I712004]), AID [http://partsregistry.org/Part:BBa_K103001 BBa_K103001])and hGH polyadenylation signal sequence ([http://partsregistry.org/Part:BBa_K404108 BBa_K404108]).
AID:
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
CMV promoter:
CMV is an immediate-early Cytomegalovirus promoter for high-level expression. The CMV promoter is commonly used due to its very strong activity and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.
hGH polyadenylation signal sequence:
Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation process.

Sequence and Features