| Line 13: | Line 13: | ||
used in the PCR. | used in the PCR. | ||
| - | We used Macherey-Nagel's "Nucleospin& | + | We used Macherey-Nagel's "Nucleospin® Gel and PCR clean-up" kit. |
| - | Its manual can be found here: | + | Its manual can be found here: [http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA%20clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf Gel and PCR clean-up Manual] |
{{:Team:EPF-Lausanne/Template/ProtocolFooter}} | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | ||
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | ||
After doing a PCR, the resulting DNA should be cleaned up to get
rid of the primers, the enzymes and the various other reagents
used in the PCR.
We used Macherey-Nagel's "Nucleospin® Gel and PCR clean-up" kit. Its manual can be found here: [http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA%20clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf Gel and PCR clean-up Manual]
"
