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Team:Trieste/parts/8
From 2012.igem.org
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<h2>Assembly</h2> | <h2>Assembly</h2> | ||
<center><img src="https://static.igem.org/mediawiki/2012/e/ec/Giulio_3.gif" width="500px"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/e/ec/Giulio_3.gif" width="500px"/></center> | ||
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<h2>Results</h2> | <h2>Results</h2> | ||
<center><img src="https://static.igem.org/mediawiki/2012/c/c0/TsTse2021.jpg" width="600px"/></center></br> | <center><img src="https://static.igem.org/mediawiki/2012/c/c0/TsTse2021.jpg" width="600px"/></center></br> | ||
Revision as of 08:53, 25 September 2012
BBa_K875008
More
Description
The Tse2 protein is a toxin component of a toxin-immunity system and arrests the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cell.
Assembly
Results
M15 bacteria cells (containing the pREP4 plasmid coding for the Lac repressor) are transformed with this Biobrick that resides on pSB1C3 plasmid. After an over night liquid culture (LB broth) the OD600 absorbance is recorded and the culture is diluted to 0,1 and the batch is splitted into 6 flask – 20ml each. Three of these are induced with IPTG 1mM and the others are not induced. The OD 600 is then recorded each hour. It is possible to observe that not induced cells growth up to OD 1, instead the induced ones do not growth during the time, demonstrating the inducible promoter and toxin efficiency.
Modelling
Looking forward
In the final system, the toxin should be under the control of the T5 Cumate Operator (BBa_K875001).
Link to the Registry
