"
Team:Trieste/parts/6
From 2012.igem.org
(Difference between revisions)
Samarisara (Talk | contribs) |
|||
| Line 11: | Line 11: | ||
<h2>Description </h2> | <h2>Description </h2> | ||
</br> | </br> | ||
| + | This construct is designed for the expression of secreted, already described engineered antinorovirus (NoV) monoclonal antibody (mAb 54.6). | ||
| + | <br/> | ||
| + | <br/> | ||
| + | The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separated by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins. | ||
| + | <br/> | ||
| + | <br/> | ||
| + | The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015). | ||
| + | <br/> | ||
| + | <br/> | ||
<h2>Assembly</h2> | <h2>Assembly</h2> | ||
</br> | </br> | ||
Revision as of 18:52, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
BBa_K875006
More
Description
This construct is designed for the expression of secreted, already described engineered antinorovirus (NoV) monoclonal antibody (mAb 54.6).The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separated by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins.
The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015).
Assembly
Results
Modelling
Looking forward
Link to the Registry
