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| | <div class="col col23"> | | <div class="col col23"> |
| | <h2>Welcome To TTEC!</h2> | | <h2>Welcome To TTEC!</h2> |
| - | <p>We developed the Transcriptional Terminator Efficiency Calculator.(TTEC)</p> | + | <p>We developed the Transcriptional Terminator Efficiency Calculator(TTEC).</p> |
| | <p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical modeling and experiment method. We first developed a theoretical model. This model calculate the free energy of RNA folding and can predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can calculate terminator efficiency. In the aspect of experiment, we construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators. The efficiency calculated from theoretical model fit quite well with experimental results.</p> | | <p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical modeling and experiment method. We first developed a theoretical model. This model calculate the free energy of RNA folding and can predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can calculate terminator efficiency. In the aspect of experiment, we construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators. The efficiency calculated from theoretical model fit quite well with experimental results.</p> |
| | <p> We also created a software and a web surver for people to calculate their terminators and also built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency. | | <p> We also created a software and a web surver for people to calculate their terminators and also built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency. |
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| | <h2>Have a Try! </h2> | | <h2>Have a Try! </h2> |
| | <ul class="tmo_ul_list"> | | <ul class="tmo_ul_list"> |
| - | <li><a href="http://www.terminatorefficiency.com/"><h3>TTEC-Web Tool</h3></a></li> | + | <li><a href="http://www.terminatorefficiency.com/"><h3>TTEC Web Tool</h3></a></li> |
| | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial"><h3>TTEC Tutorial</h3></a></li> | | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial"><h3>TTEC Tutorial</h3></a></li> |
| | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment"><h3>User Comments</h3></a></li> | | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment"><h3>User Comments</h3></a></li> |
Welcome To TTEC!
We developed the Transcriptional Terminator Efficiency Calculator(TTEC).
We studied the rho-independent transcription terminators which regulate gene expression using both theoretical modeling and experiment method. We first developed a theoretical model. This model calculate the free energy of RNA folding and can predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can calculate terminator efficiency. In the aspect of experiment, we construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators. The efficiency calculated from theoretical model fit quite well with experimental results.
We also created a software and a web surver for people to calculate their terminators and also built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.
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