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Team:LMU-Munich/Data/Inducible
From 2012.igem.org
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| - | [[File:plate reader induzierbar promotor.png|thumb|right|400px|<p align="justify">'''Fig. 1: Luminescence measurements of the promoter P<sub>''liaI''</sub> after induction with different concentrations of | + | [[File:plate reader induzierbar promotor.png|thumb|right|400px|<p align="justify">'''Fig. 1: Luminescence measurements of the promoter P<sub>''liaI''</sub> after induction with different concentrations of bacitracin.'''</p> ]] |
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The inducible promoter '''P<sub>''liaI''</sub>''' was evaluated in the reporter vector pSB<sub>Bs</sub>3C-<i>luxABCDE</i> which contains the ''lux'' operon [[File:Lux operon.png|100px]]. This is why the promoter activity leads to gene expression and to the production of the protein luciferase. The luminescence of this protein can be measured with the plate reader ''Synergy2'' (Biotek) ('''Fig.1'''). </p> | The inducible promoter '''P<sub>''liaI''</sub>''' was evaluated in the reporter vector pSB<sub>Bs</sub>3C-<i>luxABCDE</i> which contains the ''lux'' operon [[File:Lux operon.png|100px]]. This is why the promoter activity leads to gene expression and to the production of the protein luciferase. The luminescence of this protein can be measured with the plate reader ''Synergy2'' (Biotek) ('''Fig.1'''). </p> | ||
| - | [[File:Englisch Auswertung PliaI.png|thumb|right|400px|<p align="justify">'''Fig. 2: β-galactosidase assay of the inducible promoter <sub>''liaI''</sub> with and without induction with bacitracin'''</p>]] | + | [[File:Englisch Auswertung PliaI.png|thumb|right|400px|<p align="justify">'''Fig. 2: β-galactosidase assay of the inducible promoter <sub>''liaI''</sub> with and without induction with bacitracin.'''</p>]] |
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| - | The inducible promoter P<sub>''liaI''</sub>was also evaluated with the reporter vector pSB<sub>''Bs''</sub>1C-''lacZ'' which contains the ''lacZ'' reporter gene [[File:LacZ.png|50px]] ('''Fig.2'''). Promoter activity leads to the expression of the β-galactosidase which directly correlates to the promoter activity. The β-galactosidase assay of the inducible ''Bacillus'' promoter P<sub>''liaI''</sub> was repeated three times. Data show one representative result. | + | The inducible promoter P<sub>''liaI''</sub> was also evaluated with the reporter vector pSB<sub>''Bs''</sub>1C-''lacZ'' which contains the ''lacZ'' reporter gene [[File:LacZ.png|50px]] ('''Fig.2'''). Promoter activity leads to the expression of the β-galactosidase which directly correlates to the promoter activity. The β-galactosidase assay of the inducible ''Bacillus'' promoter P<sub>''liaI''</sub> was repeated three times. Data show one representative result. |
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Revision as of 18:17, 23 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Inducible Bacillus Promoters
Fig. 1: Luminescence measurements of the promoter PliaI after induction with different concentrations of bacitracin.
The inducible promoter PliaI was evaluated in the reporter vector pSBBs3C-luxABCDE which contains the lux operon 
. This is why the promoter activity leads to gene expression and to the production of the protein luciferase. The luminescence of this protein can be measured with the plate reader Synergy2 (Biotek) (Fig.1).
Fig. 2: β-galactosidase assay of the inducible promoter liaI with and without induction with bacitracin.
The inducible promoter PliaI was also evaluated with the reporter vector pSBBs1C-lacZ which contains the lacZ reporter gene 
(Fig.2). Promoter activity leads to the expression of the β-galactosidase which directly correlates to the promoter activity. The β-galactosidase assay of the inducible Bacillus promoter PliaI was repeated three times. Data show one representative result.
