Team:WashU/Protocols/PCR

From 2012.igem.org

(Difference between revisions)
Line 17: Line 17:
#1 cycle 94 degrees (3 minutes)
#1 cycle 94 degrees (3 minutes)
#40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
#40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
 +
 +
<div align="center">
 +
<font size ="5">
 +
[https://2012.igem.org/Team:WashU/Protocols Back to Protocols]

Revision as of 14:44, 22 June 2012


General PCR Protocol

Set up PCR reaction tube

  1. 1uL Template
  2. 1uL 25mM dNTP's
  3. 10uL Buffer
  4. 0.5uL Forward Primer
  5. 0.5uL Reverse Primer
  6. 0.5uL TAQ polymerase
  7. 36.5uL diH2O

Amplification reaction in thermocycler

  1. 1 cycle 94 degrees (3 minutes)
  2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)

Back to Protocols

Retrieved from "http://2012.igem.org/Team:WashU/Protocols/PCR"