Team:WashU/Protocols/6803media

From 2012.igem.org

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<!--Thanks to iGEM Harvard 2006 for the above instructions.  The original source can be found here: http://openwetware.org/wiki/IGEM:Harvard/2006/Cyanobacteria/Protocols#Mediahttp://www.neb.com/nebecomm/products_intl/protocol658.asp-->
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#Add 20 mL 50x BG-11 and 1 mM thiosulfate (= 0.248 g), top off with H20 to 1L total volume
#Add 20 mL 50x BG-11 and 1 mM thiosulfate (= 0.248 g), top off with H20 to 1L total volume
#Filter the solution into the desired container with a 0.2 µm filter.
#Filter the solution into the desired container with a 0.2 µm filter.
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==Source==
 
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Thanks to iGEM Harvard 2006 for the above instructions.  The original source can be found here: [http://openwetware.org/wiki/IGEM:Harvard/2006/Cyanobacteria/Protocols#Mediahttp://www.neb.com/nebecomm/products_intl/protocol658.asp]
 

Revision as of 19:46, 19 June 2012



Synechocystis 6803 Media

1L agar for freshwater cyanobacteria (no glucose)

  1. Mix 10 g agar and 1 mM thiosulfate (= 0.248 g), top off with H20 to 500 mL total volume.
  2. Autoclave the product of (1)
  3. Mix 20mL 50x BG-11 solution and 480 mL of H20
  4. Autoclave the product of (3)
  5. Mix (2) and (4), pour plates and let cool

1L agar for freshwater cyanobacteria (with glucose)

  1. Mix 10 g agar and 1mM thiosulfate (= 0.248 g), top off with H20 to 500 mL total volume
  2. Autoclave the product of (1)
  3. Mix 20 mL 50x BG-11 solution and 230 mL of H20
  4. Autoclave the product of (3)
  5. Mix 5 mL glucose and 245 mL of H20. [2]
  6. Autoclave the product of (5)
  7. Mix (2) and (4) and (6), pour plates and let cool


1L liquid media for freshwater cyanobacteria

  1. Add 20 mL 50x BG-11 and 1 mM thiosulfate (= 0.248 g), top off with H20 to 1L total volume
  2. Filter the solution into the desired container with a 0.2 µm filter.
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