Team:USP-UNESP-Brazil/Plasmid Plug n Play/Modeling

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<h1 id="objective">Objective</h1>
<h1 id="objective">Objective</h1>
<p>In order to evaluate the feasibility of our project, we developed a mathematical model based on kinetic equations to simulate our experimental design. We considered important to approach this problem mathematically in order to evaluate some issues. Firstly, we evaluated the effect of linear DNA degradation of the ORF when inserted in bacteria after eletroporation. Secondly, we estimated the amount of ORF that should be amplified by PCR in order to optimize the recombination. We compared the results obtained using two recombination protein: CRE and FLP. Finally, we discuss methodologies to improve our design using the standard biological parts.</p>
<p>In order to evaluate the feasibility of our project, we developed a mathematical model based on kinetic equations to simulate our experimental design. We considered important to approach this problem mathematically in order to evaluate some issues. Firstly, we evaluated the effect of linear DNA degradation of the ORF when inserted in bacteria after eletroporation. Secondly, we estimated the amount of ORF that should be amplified by PCR in order to optimize the recombination. We compared the results obtained using two recombination protein: CRE and FLP. Finally, we discuss methodologies to improve our design using the standard biological parts.</p>

Revision as of 17:18, 23 September 2012