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| - | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}}
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| - | ==Use of Antibiotic Stocks==
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| - | Stocks are to be kept in a -20<sup>o</sup>c freezer, clearly labelled.
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| - | Available stocks in the lab are:
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| - | *Ampicillin 100mg/ml , 1ml in each eppendorf
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| - | *Chloramphenicol 25mg/ml , 200µl in each eppendorf
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| - | *Kanamycin 50mg/ml , 1ml in each eppendorf
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| - | *Erythromycin 10mg/ml , 1ml in each eppendorf
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| - | ===To make plates:===
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| - | Put one entire tube into 1 litre of LB AGAR - This should be done in the flow hood.
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| - | This will make the ''standard plates''
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| - | *Ampicillin 100µg/ml
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| - | *Chloramphenicol 5µg/ml
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| - | *Kanamycin 50µg/ml
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| - | *Erythromycin 5µg/ml
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| - | To make these concentrations in the standard 250ml bottle LB Agar, add:
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| - | *250µl ampicillin stock
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| - | *50µl chloramphenicol stock
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| - | *250µl kanamycin stock
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| - | *125µl erythromycin stock
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| - | ===Common plate concentrations for backbones:===
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| - | *pSB1C3 (''E.coli'') : 25µg/ml Chloramphenicol
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| - | *pSJ150 (''E.coli'') : 100µg/ml Ampicillin
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| - | *pSJ150 (''B.subtilis'') : 5µg/ml Chloramphenicol
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| - | *pSB1K3 (''E.coli'') : 50µg/ml Kanamycin
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| - | *PSB4K5 (''E.coli'') : 50µg/ml Kanamycin
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| - | ===To make liquid cultures===
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| - | Check for specific concentrations, this is usually lower than the ones you use for plates, and add the required amount to sterile LB.
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| - | <center>'''[[Team:Cambridge/Protocols|Back to Protocols]]'''</center>
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| - | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}}
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