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Team:SDU-Denmark/labwork/Notebook/week4
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Revision as of 13:15, 23 September 2012
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
23-07-2012:
SST send for sequencing
SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.
24-07-2012:
Cryo backup, miniprep and preparation to sequencing
We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that three samples had bands corresponding to our cut gene and vector.
These will be shipped of to be sequneced tomorrow.
24-07-2012:
SST send for sequencing
Dato 25/7-12 Kristian og Lars Sample 2.3 and 2.6 was prepared for sequencing by diluting them 6:15 giving a total of around 70-78 ng/uL and sent them for sequencing. Afterward we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing. Lars will upload the work when it is done (between now and the weekend). -results from sequencing -primers ordered
