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Team:Carnegie Mellon/Met-Notebook
From 2012.igem.org
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<b> Links to Notebooks</b></h2> | <b> Links to Notebooks</b></h2> | ||
| - | 1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming | + | 1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design |
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| - | 2. <a href="https://docs.google.com/document/d/1Hf4BhpGf1OCukNzULbElX0ZgVzSmGa7Fu4kH8AxSxE8/edit">Week 4</a> was focused on testing the functionality of the FAP | + | 2. <a href="https://docs.google.com/document/d/1Hf4BhpGf1OCukNzULbElX0ZgVzSmGa7Fu4kH8AxSxE8/edit">Week 4</a> was focused on testing and comparing the functionality of the two FAP plasmids expressed in BL21DE3 cells using two variants of Malachite Green (5% EtOH and PBS) |
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| - | 3. <a href="https://docs.google.com/document/d/1r4RECG92rop1xuOs-0jIC303CcVzAKN3bNZLa1iaxvw/edit">Week 5</a> | + | 3. <a href="https://docs.google.com/document/d/1r4RECG92rop1xuOs-0jIC303CcVzAKN3bNZLa1iaxvw/edit">Week 5</a> was focused on cloning our synthesized Spinach cassette into our vector, and conducting dosage and temporal curves using the FAP and MG(5% EtOH) used. |
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| - | 4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> | + | 4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies. |
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5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> | 5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> | ||
Revision as of 03:57, 23 September 2012
Notebook
Overview
During the first 2 weeks after final exams, we were mainly focused on settling the logistics of getting the project off the ground. Hence there were no lab experiments and hence no notebook kept.
Feel free to click on the links below to view the actual lab notebooks and email us if you have any questions!
Links to Notebooks
1. Week 3 was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design2. Week 4 was focused on testing and comparing the functionality of the two FAP plasmids expressed in BL21DE3 cells using two variants of Malachite Green (5% EtOH and PBS)
3. Week 5 was focused on cloning our synthesized Spinach cassette into our vector, and conducting dosage and temporal curves using the FAP and MG(5% EtOH) used.
4. Week 6 was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies.
5. Week 7
6. Week 8
7. Week 9
8. Week 10
9. Week 11
10. Week 12
