Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(→Plasmid Database)
(→Strain Database)
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! Box
! Box
! Slot
! Slot
-
! Name
+
! Label
! Description
! Description
 +
! Strain
! Source
! Source
 +
! Added by
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
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| AG1
| AG1
| Host strain of ASKA collection
| Host strain of ASKA collection
 +
|
| Barrick Lab
| Barrick Lab
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
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| BW25113
| BW25113
| Host strain of Keio collection
| Host strain of Keio collection
 +
|
| Barrick Lab
| Barrick Lab
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
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| cadA
| cadA
| AG1 + ASKA collection cadA plasmid, CamR
| AG1 + ASKA collection cadA plasmid, CamR
 +
|
| Barrick Lab
| Barrick Lab
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
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| ldcC
| ldcC
| AG1 + ASKA collection ldcC plasmid, CamR
| AG1 + ASKA collection ldcC plasmid, CamR
 +
|
| Barrick Lab
| Barrick Lab
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
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| tnaA
| tnaA
| Keio collection knockout (version of odor-less E. coli), KanR
| Keio collection knockout (version of odor-less E. coli), KanR
 +
|
| Barrick Lab
| Barrick Lab
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
| 7
| 7
| IGM009
| IGM009
-
| crdASC (4864 bp) in TOP10
+
| crdASC (4864 bp)
 +
| TOP10
| Barrick Lab
| Barrick Lab
 +
| Ben
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
| 8
| 8
| IGM010
| IGM010
-
| crdASC (4943 bp) in TOP10
+
| crdASC (4943 bp)
 +
| TOP10
| Barrick Lab
| Barrick Lab
 +
| Ben
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
| 9
| 9
| IGM011
| IGM011
-
| crdASC (4864 bp) in BL21(DE3)
+
| crdASC (4864 bp)
 +
| BL21(DE3)
| Barrick Lab
| Barrick Lab
 +
| Ben
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
| 10
| 10
| IGM012
| IGM012
-
| crdASC (4943 bp) in BL21(DE3)
+
| crdASC (4943 bp)
 +
| BL21(DE3)
| Barrick Lab
| Barrick Lab
-
|  
+
| Ben
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
| 11
| 11
| IGM013
| IGM013
-
| BBa_K515105: J23100-sfGFP-pSB1C3(CamR) in epi300
+
| BBa_K515105: J23100-sfGFP-pSB1C3(CamR)
 +
| epi300
| Parts Kit
| Parts Kit
-
 
+
|
 +
|-
|}
|}

Revision as of 21:24, 19 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Label Name Part Plasmid Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 IGM004 GFP [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L)
iGEM 2012 Plasmid Box 1 IGM005 RFP [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O)
iGEM 2012 Plasmid Box 1 IGM006 LasI [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N)
iGEM 2012 Plasmid Box 1 IGM007 RBS + LacI [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679] pSB1A2 2012 kit plate 1 (7L)
iGEM 2012 Plasmid Box 1 IGM008 Double terminator (BBa_B0010-BBa_B0012) [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] pSB1AK3 2012 kit plate 1 (23L)
iGEM 2012 Plasmid Box 1 IGM009 J23100 promoter - sfGFP (Part:BBa_K515105) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] pSB1C3 2012 kit plate 5 (1K)

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Label Description Strain Source Added by
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 7 IGM009 crdASC (4864 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 8 IGM010 crdASC (4943 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 9 IGM011 crdASC (4864 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 10 IGM012 crdASC (4943 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 11 IGM013 BBa_K515105: J23100-sfGFP-pSB1C3(CamR) epi300 Parts Kit

ZombiE.coli parts

Plasmid Part Description Source
pReporter [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] RFP protein generator - PoPS->RFP 2012 kit plate 3 (12O)
pReporter [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS + LacI 2012 kit plate 1 (7L)
pReporter [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] LasR Promoter 2012 kit plate 1 (12A)
pConverter [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] LasR 2012 kit plate 1 (14J)
pConverter [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] LacIM1 Parts Registry
pConverter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"