Team:Goettingen/week12-3

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<b>V07_17_1 Test digestion of Miniprep V07_12 </b><br>
<b>V07_17_1 Test digestion of Miniprep V07_12 </b><br>
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<li>Experiment: <br>A test digestion using EcoRI and PstI in a 20 µL batch was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods>protocol</a> to confirm that the mutant plasmid pSB1C3_TAR_QC_18C has actually been isolated correctly. </li>
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<li>Experiment: <br>A test digestion using EcoRI and PstI in a 20 µL batch was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> to confirm that the mutant plasmid pSB1C3_TAR_QC_18C has actually been isolated correctly. </li>
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Revision as of 18:47, 22 September 2012

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#3 Chemoreceptor Library - 12th Week

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V07_16


Starpoint of the 2nd round of saturated mutagenesis PCR!
  • Experiment:
    For the second round of saturated mutagenesis PCR we used the primers Tar6973Fw and Tar6973Rv. Here, aa residues 69 and 73 will be mutated. The PCR protocol is basically the same as for the first round.
  • Observations and results:
    The corresponding agarose gel showed a strong band of the expected size (3769 bp).


V07_17


V07_17_1 Test digestion of Miniprep V07_12
  • Experiment:
    A test digestion using EcoRI and PstI in a 20 µL batch was performed according to protocol to confirm that the mutant plasmid pSB1C3_TAR_QC_18C has actually been isolated correctly.
  • Observations and results:
    The corresponding agarose gel showed two distinctive bands of the expected sizes which led to the conclusion that the plasmid had indeed been isolated!

V07_17_2 2nd round: saturated mutagenesis PCR, volume 1 mL
  • Experiment:
  • Observations and results:


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